PPP1R13L polyclonal antibody
产品名称： PPP1R13L polyclonal antibody
英文名称： PPP1R13L polyclonal antibody
产品编号： PAB10243
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PPP1R13L.
- Immunogen:
- A synthetic peptide corresponding to amino acids 780-797 of human PPP1R13L.
- Host:
- Rabbit
- Reactivity:
- Bovine, Dog, Human, Mouse
- Specificity:
- This affinity-purified antibody is directed against the human iASPP protein. Expect reactivity against both isoform 1 and isoform 2.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:40000-1:160000)
Western Blot (1:1000-1:5000)
Immunohistochemistry (1:1000-1:5000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- ASPP--Apoptotic specific regulator of p53.
Liu ZJ, Lu X, Zhong S.Biochim Biophys Acta. 2005 Sep 25;1756(1):77-80.
- 2.
- Polymorphisms in RAI and in genes of nucleotide and base excision repair are not associated with risk of testicular cancer.
Laska MJ, Nexo BA, Vistisen K, Poulsen HE, Loft S, Vogel U.Cancer Lett. 2005 Jul 28;225(2):245-51.
- 3.
- The expression of iASPP in acute leukemias.
Zhang X, Wang M, Zhou C, Chen S, Wang J.Leuk Res. 2005 Feb;29(2):179-83.
- 4.
- The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization.
Slee EA, Gillotin S, Bergamaschi D, Royer C, Llanos S, Ali S, Jin B, Trigiante G, Lu X.Oncogene. 2004 Dec 2;23(56):9007-16.
- 5.
- RelA-associated inhibitor blocks transcription of human immunodeficiency virus type 1 by inhibiting NF-kappaB and Sp1 actions.
Takada N, Sanda T, Okamoto H, Yang JP, Asamitsu K, Sarol L, Kimura G, Uranishi H, Tetsuka T, Okamoto T.J Virol. 2002 Aug;76(16):8019-30.
- Applications
- Western Blot (Cell lysate)
- Western blot using PPP1R13L polyclonal antibody (Cat # PAB10243) shows detection of a band at ~100 KDa (arrowhead) corresponding to isoform 1 of PPP1R13L in MCF-7 whole cell lysates.
Preincubation with immunizing peptide blocks specific band staining (data not shown).
Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4°C.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry of PPP1R13L polyclonal antibody (Cat # PAB10243) shows strong cytoplasmic and membranous staining of myocytes in human heart tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
- Entrez GeneID:
- 10848
- Protein Accession#:
- NP_006654;Q8WUF5
- Gene Name:
- PPP1R13L
- Gene Alias:
- IASPP,NKIP1,RAI
- Gene Description:
- protein phosphatase 1, regulatory (inhibitor) subunit 13 like
- Omim ID:
- 607463
- Gene Ontology:
- Hyperlink
- Gene Summary:
- IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM
- Other Designations:
- NFkB interacting protein 1,RelA-associated inhibitor,inhibitor of apoptosis stimulating protein of p53,protein phosphatase 1, regulatory subunit 13 like