PML monoclonal antibody (M02), clone 1D12
产品名称： PML monoclonal antibody (M02), clone 1D12
英文名称： PML monoclonal antibody (M02), clone 1D12
产品编号： H00005371-M02
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant PML.
- Immunogen:
- PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- RDPIDVDLDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVV
- Host:
- Mouse
- Reactivity:
- Human, Rat
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- PML monoclonal antibody (M02), clone 1D12. Western Blot analysis of PML expression in PC-12(Cat # L012V1 ).
- Protocol Download
- Western Blot (Cell lysate)
- PML monoclonal antibody (M02), clone 1D12 Western Blot analysis of PML expression in Hela NE ( Cat # L013V3 ).
- Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged PML is approximately 0.3ng/ml as a capture antibody.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between TP53 and PML HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PML mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Entrez GeneID:
- 5371
- GeneBank Accession#:
- BC000080
- Protein Accession#:
- AAH00080
- Gene Name:
- PML
- Gene Alias:
- MYL,PP8675,RNF71,TRIM19
- Gene Description:
- promyelocytic leukemia
- Omim ID:
- 102578
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
- Other Designations:
- promyelocytic leukemia protein,promyelocytic leukemia, inducer of,tripartite motif protein TRIM19