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产品：猪视网膜色素上皮细胞

货号：P6540

Description

The retina is a multi-layered structure lining the rear of the eye. Itconsists of photoreceptor cells and retinal pigment epithelial cells(RPE). RPE situated between the neurosensory retina and the choroid,form the outer blood-retina barrier that controls the chemicalcomposition of the subretinal space [1]. RPE has long been recognized todiffer from many other epithelia in the polarity of a number of proteinsand whereas almost all other epithelia face an apical lumen devoid ofmatrix, the RPE apical plasma membrane is in contact with theextracellular matrix [2]. During early embryonic development, RPEspontaneously transdifferentiate into neural retinal tissue [3]. RPEplay a critical role in the development and maintenance of adjacentphotoreceptors. They generate a number of reactive oxygen species whenilluminated with light and their proliferation is an important step inthe pathogenesis of ocular diseases such as proliferative retinopathy.

PRPEpiC from ScienCell Research Laboratories are isolated from porcineretina. PRPEpiC are cryopreserved at passage one and delivered frozen.Each vial contains >5 x 10^5 cells in 1 ml volume. PRPEpiC arecharacterized by immunofluorescent method with antibodies tocytokeratin-18, cytokeratin-19 and fibronectin. PRPEpiC are negative forHIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. PRPEpiC areguaranteed to further expand for 15 population doublings in thecondition provided by ScienCell Research Laboratories.


Recommended Medium

It is recommended to use Epithelial Cell Medium (EpiCM, Cat No. 1401)for expanding PRPEpiC in vitro.


Product Use

PRPEpC are for research use only. It is not approved for human oranimal use, or for application in in vitro diagnostic procedures.


Storage

Directly and immediately transfer cells from dry ice to liquid nitrogenupon receiving and keep the cells in liquid nitrogen until cell cultureneeded for experiments.


Shipping

Dry ice.


Reference

[1] Rizzolo, L. J. (1997) Polarity and the development of the outerblood-retinal barrier. Histol Histopathol 12:1057-1067.
[2] Marmorstein, A. D. (2001) The polarity of the retinal pigmentepithelium. Traffic 2:867-872.
[3] Araki, M., Takano, T., Uemonsa, T., Nakane, Y., Tsudzuki, M.,Kaneko, T. (2002) Epithelia-mesenchyme interaction plays an essentialrole in transdifferentiation of retinal pigment epithelium of silvermutant quail: localization of FGF and related molecules and aberrantmigration pattern of neural crest cells during eye rudiment formation.Dev Biol. 244:358-71.