含杆状病毒IAP重复蛋白6(BIRC6)重组蛋白

YB233Hu015
Recombinant Baculoviral IAP Repeat Containing Protein 6 (BIRC6)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
 含杆状病毒IAP重复蛋白6(BIRC6)重组蛋白Source: Prokaryotic expression. Host: E. coli
Residues: Arg4576~Leu4857
Tags: N-terminal His-Tag
Tissue Specificity: Brain. Subcellular Location: Golgi apparatus, trans-Golgi network membrane. Midbody. Endosome. Cytoplasm, cytoskeleton, spindle pole. Cytoskeleton, microtubule
organizing center, centrosome. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
 含杆状病毒IAP重复蛋白6(BIRC6)重组蛋白Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.4
Predicted Molecular Mass: 35.5kDa
Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as
follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
 含杆状病毒IAP重复蛋白6(BIRC6)重组蛋白oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE