GART monoclonal antibody (M01), clone 4D6-1D5
产品名称： GART monoclonal antibody (M01), clone 4D6-1D5
英文名称： GART monoclonal antibody (M01), clone 4D6-1D5
产品编号： H00002618-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant GART.
- Immunogen:
- GART (AAH38958.1, 1 a.a. ~ 433 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTKNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTKGVEITGFPEAQALGLEVFHAGTALKNGKVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKDVGFRAIAFLQQPR
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (73.37 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.
Noelker C, Schwake M, Balzer-Geldsetzer M, Bacher M, Popp J, Schlegel J, Eggert K, Oertel WH, Klockgether T, Dodel R.Neurosci Lett. 2011 Dec 1.
- 2.
- No Evidence for Mutations that Deregulate GARS?VAIRS?VGART Protein Levels in Children with Down Syndrome.
Banerjee D, Ghosh D, Chatterjee A, Sinha S, Nandagopal K.Ind J Clin Biochem DOI 10.1007/s12291 -011-0183-6
- 3.
- 5,10-Methenyltetrahydrofolate Synthetase Activity is Increased in Tumors and Modifies the Efficacy of Antipurine LY309887.
Field MS, Anguera MC, Page R, Stover PJ.Arch Biochem Biophys. 2009 Jan 15;481(2):145-50. Epub 2008 Nov 8.
- Applications
- Western Blot (Cell lysate)
- GART monoclonal antibody (M01), clone 4D6-1D5 Western Blot analysis of GART expression in HeLa NE ( Cat # L013V3 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of GART expression in transfected 293T cell line by GART monoclonal antibody (M01), clone 4D6-1D5.
Lane 1: GART transfected lysate(46 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 2 ug/ml]
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to GART on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of GART transfected lysate using anti-GART monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GART MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged GART is approximately 0.3ng/ml as a capture antibody.
- Protocol Download
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunofluorescence
- enlarge
- Immunoprecipitation
- enlarge
- Sandwich ELISA (Recombinant protein)
- ELISA
- Entrez GeneID:
- 2618
- GeneBank Accession#:
- BC038958
- Protein Accession#:
- AAH38958.1
- Gene Name:
- GART
- Gene Alias:
- AIRS,GARS,GARTF,MGC47764,PAIS,PGFT,PRGS
- Gene Description:
- phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
- Omim ID:
- 138440
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
- Other Designations:
- OTTHUMP00000068280,OTTHUMP00000068281,OTTHUMP00000068282,OTTHUMP00000068337,trifunctional purine biosynthetic protein adenosine-3