<h1 style="color: #3c506c;"><span style="color: #f79646;"><strong>为了提升客户使用体验，YeaRed的产品管由2 mL棕色管改换成0.5 mL的棕色管，有利于减少贴壁作用！</strong></span></h1> <section style="color: #3c506c;" data-role="outer"> <p align="justify"><span style="font-family: Helvetica Neue宋体;">YeaRed是一种独特的油性大分子，不能穿透细胞膜进入细胞内。不易挥发升华，人体不会吸入。艾姆斯氏测试结果也表明YeaRed在凝胶染色浓度下完全无诱变性，是一种安全无毒的核酸染料。YeaRed与EB具有相同的光谱特性，无需改变滤光片及观察装置（普通紫外凝胶透射仪），在300 nm处紫外光激发检测即可。YeaRed适用于琼脂糖和聚丙烯酰胺凝胶电泳中的dsDNA，ssDNA以及RNA染色，可以选择胶染法或泡染法进行染色，使用非常方便、灵活。</span></p> <p align="justify">&nbsp;</p> <p align="justify"><strong><span style="color: #f79646;"><span style="font-family: Helvetica Neue宋体;">储存条件</span></span></strong></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">避光室温保存，有效期</span><span style="font-family: Helvetica Neue宋体;">5年。</span></p> <p align="justify">&nbsp;</p> <p align="justify"><strong><span style="color: #f79646;"><span style="font-family: Helvetica Neue宋体;">使用方法</span></span></strong></p> <p>1.<span style="font-family: Helvetica Neue宋体;">胶染法（同</span><span style="font-family: Helvetica Neue宋体;">EB，电泳前染色）</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">1</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">配制合适浓度的琼脂糖凝胶，微波炉加热至完全熔化。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">2</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">加入</span><span style="font-family: Helvetica Neue宋体;">YeaRed核酸染料，使用终浓度为1&times;（即每50 mL琼脂糖溶液中加入5 &mu;L YeaRed 10,000&times;水溶液）。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">3</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">将含有</span><span style="font-family: Helvetica Neue宋体;">YeaRed核酸染料的琼脂糖溶液倒入制胶器并插好梳子，室温下凝固约30-60 min。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">4</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">按照常规方法上样并电泳。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">5</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">紫外拍照观察。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">【注】：</span><span style="font-family: Helvetica Neue宋体;">YeaRed具有良好的热稳定性，可以在热的琼脂糖溶液中直接添加，也可以将YeaRed储液加到含有琼脂糖粉末的电泳缓冲液中，然后用微波炉或其他常用方式加热以制备琼脂糖凝胶。YeaRed兼容所有常用的电泳缓冲溶液。</span></p> <p>2.<span style="font-family: Helvetica Neue宋体;">泡染法（电泳后染色）</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">1</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">配制合适浓度的琼脂糖凝胶，微波炉加热至完全熔化。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">2</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">将琼脂糖溶液倒入制胶器并插好梳子，室温下凝固约</span><span style="font-family: Helvetica Neue宋体;">30-60 min。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">3</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">按照常规方法上样并电泳。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">4</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">用</span><span style="font-family: Helvetica Neue宋体;">0.1 M NaCl溶液稀释YeaRed 10,000&times;水溶液至3&times;染色液（即将15 &mu;L YeaRed 10,000&times;水溶液加入到50 mL 0.1 M NaCl溶液中，该染液可重复使用3次左右，室温避光保存）。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">5</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">将凝胶放入合适的容器中，加入</span><span style="font-family: Helvetica Neue宋体;">3&times;染色液浸没凝胶。室温振荡染色30 min左右。最佳染色时间与凝胶厚度及浓度有关。对于含3.5</span><span style="font-family: Helvetica Neue宋体;">-</span><span style="font-family: Helvetica Neue宋体;">10％聚丙烯酰胺的凝胶，染色时间通常介于30 min到1 h，并随聚丙烯酰胺含量增加而延长。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">6</span><span style="font-family: Helvetica Neue宋体;">）</span><span style="font-family: Helvetica Neue宋体;">紫外拍照观察。</span></p> <p align="justify">&nbsp;</p> <p align="justify"><strong><span style="color: #f79646;"><span style="font-family: Helvetica Neue宋体;">注意事项</span></span></strong></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">1. 若大分子条带拖尾且分离不理想，建议减少DNA marker或核酸样本的上样量。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">2. 胶染法不适合预制聚丙烯酰胺凝胶，对于聚丙烯酰胺凝胶请使用泡染法。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。</span></p> <p align="justify"><span style="font-family: Helvetica Neue宋体;">4. 本产品仅作科研用途！</span></p> <p><span style="font-family: Helvetica Neue宋体;">Ver.CN</span><span style="font-family: Helvetica Neue宋体;">2</span><span style="font-family: Helvetica Neue宋体;">0230829</span></p> </section>